All Publications

Quantitative measurement of alterations in DNA damage repair (DDR) pathways using single cell network profiling (SCNP).
Rosen DB, Leung LY, Louie B, Cordeiro JA, Conroy A, Shapira I, Fields SZ, Cesano A, and Hawtin RE.
J Transl Med 2014;12:184.

High-dimensional analysis of the aging immune system: Verification of age-associated differences in immune signaling responses in healthy donors.
Longo DM, Louie B, Ptacek J, Friedland G, Evensen E, Putta S, Atallah M, Spellmeyer D, Wang E, Pos Z, Marincola FM, Schaeffer A, Lukac S, Railkar R, Beals CR, Cesano A, Carayannopoulos LN, and Hawtin RE.
J Transl Med 2014;12:178.

Profiling signaling pathways in CLL PD-1+ CD8 T cells identifies features of “pseudo-exhaustion”.
Liang SC, Leung L, Putta S, and Hawtin R.
Presented at: Keystone Symposia: Immune Evolution in Cancer (X2). March 9–14, 2014; Whistler, British Columbia, CA.

Systems biology analysis of immune signaling in peripheral blood mononuclear cells (PBMC) of melanoma patients receiving ipilimumab; basis for response biomarker identification.
Hotson D, Alvarado R, Conroy A, Putta S, Simeone E, Capone M, Madonna G, Grimaldi AM, Liang S, Rosen D, Cesano A, Ascierto P, and Hawtin R.
Presented at: 3rd Annual Melanoma Bridge; December 5–8, 2013; Naples, IT.

Single cell network profiling (SCNP): technology and its application to the development of cancer immunotherapy.
Spellmeyer D.
Presented at: 13th Euroconference on Clinical Cell Analysis; November 12–4, 2013; Luxembourg, LU.

Systems biology analysis of immune signaling in peripheral blood mononuclear cells (PBMC) of melanoma patients receiving ipilimumab; basis for response biomarker identification.
Hotson D, Alvarado R, Conroy A, Putta S, Simeone E, Capone M, Madonna G, Grimaldi A, Liang S, Cesano A, Ascierto P, and Hawtin R.
Presented at: 28th Annual Meeting of the Society for Immunotherapy of Cancer; November 8–10, 2013; National Harbor, MD.

Prediction of TNF inhibitor response in Rheumatoid Arthritis patients using Single Cell Network Profiling of intracellular immune signaling.
Ptacek J, Hawtin R, Louie B, Evensen E, Cordeiro J, Mittleman B, Atallah M, Cesano A, Bingham C, Cofield S, Curtis J, Danila MI, Furie R, Genovese MC, Levesque MC, Moreland LW, Nigrovic PA, O'Dell JR, Robinson WH, Shadick NA, St Clair EW, et al.
Presented at: Annual Meeting of the American College of Rhematology; October 25–30, 2013; San Diego, CA.

Novel biomarkers from peripheral blood mononuclear cells indicate disease activity in Rheumatoid Arthritis patients.
Ptacek J, Hawtin R, Louie B, Evensen E, Cordeiro J, Mittleman B, Atallah M, Cesano A, Bingham C, Cofield S, Curtis J, Danila MI, Furie R, Genovese MC, Levesque MC, Moreland LW, Nigrovic PA, O'Dell JR, Robinson WH, Shadick NA, St Clair EW, et al.
Presented at: Annual Meeting of the American College of Rhematology; October 25–30, 2013; San Diego, CA.

Application of SCNP technology to autoimmunity for both disease characterization and prediction of response to therapy.
Cesano A.
Presented at: 28th Annual Meeting of the International Clinical Cytometry Society; October 11–5, 2013; Fort Lauderdale, FL.

Validation of cell-based fluorescence assays: Practice guidelines from the ICSH and ICCS - part III - analytical issues.
Tanqri S, Vall H, Kaplan D, Hoffman B, Purvis N, Porwit A, Hunsberger B, and Shankey TV.
Cytometry Part B 2013; 84(5).

Age-related changes of healthy bone marrow cell signaling in response to growth factors provide insight into low risk MDS.
Kornblau SM, Cohen AC, Soper D, Huang YW, and Cesano A.
Cytometry Part B 2013; doi: 10.1002/cytob.21125.

Comparison of functional immune signaling profiles in peripheral blood mononuclear cells (PBMC) from rheumatoid arthritis (RA) patients versus healthy donors (HD) using Single Cell Network Profiling (SCNP).
Ptacek J, Hawtin R, Evensen E, Cordeiro J, Atallah M, Marimpietri C, Cesano A, TETRAD Investigators, Gregersen PK, and Bridges SL.
Presented at: 15th International Congress of Immunology; August 22–7, 2013; Milan, IT.

Functional proteomic interrogation of immune cell crosstalk and the effects of cytokine-targeted inhibitors using Single Cell Network Profiling (SCNP).
Hotson D, Conroy A, Cesano A, and Hawtin R.
Presented at: 15th International Congress of Immunology; August 22–7, 2013; Milan, IT.

Vaccine hyporesponse in healthy elderly subjects is associated with a decrease in B cell IFN-α responses: Data from functional proteomic analysis using single cell network profiling.
Longo D.
Presented at: 3rd International Conference on Vaccines & Vaccination; July 29–31, 2013; Las Vegas, NV.

Single cell network profliling (SCNP) analysis reveals dysfunctional signaling within the Th17 axis in chronic lymphocytic leukemia (CLL).
Liang S, Ptacek J, Evensen E, Leung L, Stockett D, Chiorazzi N, Sherry B, and Hawtin R.
Presented at: 18th Congress of the European Hematology Association; June 13–6, 2013; Stockholm, SE.

Deregulation of stem cell factor (SCF)-AKT-S6 pathway in diagnostic AML samples is associated with disease-free survival: results of a verification study.
Cesano A, Willman CL, Kopecky KJ, Putta S, Cohen A, Marimpietri C, Atwater SK, Appelbaum FR, and Radich JP.
Presented at: 18th Congress of the European Hematology Association; June 13–6, 2013; Stockholm, SE.

Development and validation of a single-cell network profiling assay-based classifier to predict response to induction therapy in paediatric patients with de novo acute myeloid leukaemia: a report from the Children's Oncology Group.
Lacayo NJ, Alonzo TA, Gayko U, Rosen DB, Westfall M, Purvis N, Putta S, Louie B, Hackett J, Cohen AC, Cesano A, Gerbing R, Ravindranath Y, Dahl GV, Gamis A, and Meshinchi S.
British Journal of Haematology 2013; 10.1111/bjh.12370: ePub ahead of print.

Functional characterization of KIT and FcεR1 receptor mutations in Mast Cell Leukemia using Single Cell Network Profiling (SCNP).
Westfall M, Spector M, Gulrajani M, Marimpietri C, Kritharis A, Fields SZ, Allen SL, and Hawtin R.
Presented at: 104th Annual Meeting of the American Association for Cancer Research; April 6–10, 2013; Washington, DC.

Quantitative measurements of EGFR pathway signaling and modulation in pleural effusion samples from non-small cell lung cancer (NSCLC) patients using single cell network profiling (SCNP).
Westfall M, Hawtin R, Covey T, Gulrajani M, Atallah M, Cholankeril M, Vora R, Cesano A, Fields SZ, and Raftopoulos H.
Presented at: 104th Annual Meeting of the American Association for Cancer Research; April 6–10, 2013; Washington, DC.

Fighting Cancer with Python.
Evensen E.
Presented at: PyCon 2013; March 13–21, 2013; Santa Clara, CA.

Functional pathway analysis using SCNP of FLT3 receptor pathway deregulation in AML provides prognostic information independent from mutational status.
Cesano A, Putta S, Rosen DB, Cohen AC, Gayko U, Mathi K, Woronics J, Hawtin RE, Cripe L, Sun Z, Tallman MS, and Paietta E.
PLOS One 2013;8(2):e56714.

AKT signaling as a novel factor associated with in-vitro resistance of human AML to gemtuzumab ozogamicin.
Rosen DB, Harrington KH, Cordeiro JA, Leung LY, Putta S, Lacayo N, Laszlo GS, Gudgeon CJ, Hogge DE, Hawtin RE, Cesano A, and Walter RB.
PLoS ONE 2013;8(1):e53518.

Single Cell Network Profiling Assay in Bladder Cancer.
Covey TM, Vira MA, Westfall M, Gulrajani M, Cholankeril M, Okhunov Z, Levey H, Marimpietri C, Hawtin R, Fields SZ, and Cesano A.
Cytometry Part A 2013; 83(1).

Assessing germline Homologous Recombination pathway deficiency in BRCA1 mutation carriers using Single Cell Network Profiling.
Rosen DB, Leung L, Louie B, Evensen E, Fields SZ, Cesano A, Shapira I, and Hawtin R.
Presented at: 35th Annual Meeting of the San Antonia Breast Cancer Symposium (SABCS); December 4–8, 2012; San Antonio, TX.

Single cell network profiling (SCNP) identifies altered signaling between patient risk groups in B-cell chronic lymphocytic leukemia (B-CLL).
Ptacek J, Evensen E, Friedland G, Cordeiro J, Ware JR, Chu CC, Damle RN, Zhang L, Chiorazzi N, Cesano A, and Hawtin RE.
Presented at: 54th Annual Meeting of the American Society of Hematology; December 8–11, 2012; Atlanta, GA.

Single cell network profiling (SCNP) of early-stage chronic lymphocytic leukemia (CLL): Association with p53, IGHV mutational status and time to first treatment (TTFT).
Ptacek J, Evensen E, Hawtin RE, Friedland G, Ware JR, Cordeiro J, Peragine N, Cesano A, Foa R, and Guarini A.
Presented at: 54th Annual Meeting of the American Society of Hematology; December 8–11, 2012; Atlanta, GA.

Single cell network profiling (SCNP)-based classifier to predict response to induction therapy in elderly patients with acute myeloid leukemia (AML): Validation in two independent sample sets from ECOG and SWOG trials.
Cesano A, Willman CL, Kopecky KJ, Gayko U, Putta S, Louie B, Westfall M, Purvis N, Spellmeyer D, Marimpietri C, Hackett J, Shi J, Paietta E, Tallman MS, Cripe LD, Atwater SK, Appelbaum FR, and Radich JP.
Presented at: 54th Annual Meeting of the American Society of Hematology; December 8–11, 2012; Atlanta, GA.

Association between B-cell receptor responsiveness and disease progression in B-cell chronic lymphocytic leukemia: results from single cell network profiling studies.
Cesano A, Perbellini O, Evensen E, Chu CC, Cioffi F, Ptacek J, Damle RN, Chignola R, Cordeiro J, Yan X, Hawtin RE, Nichele I, Ware JR, Cavallini C, Lovato O, Zaanotti R, Rai KR, Chiorazzi N, Pizzolo G, and Scupoli MT.
Haematologica 2012;97. [ePub ahead of print]

CTLA-4 defines distinct T cell signaling populations in healthy donors and metastatic melanoma patients.
Hotson D, Conroy A, Evensen E, Gentilcore G, Simeone E, Esposito A, Curvietto M, Cesano A, Hawtin R, and Ascierto P.
Presented at: 27th Annual Meeting of the Society for Immunotherapy of Cancer; October 24–8, 2012; North Bethesda, MD.

Functional pathway analysis using single cell network profiling (SCNP) shows prognostic value in patients with B-cell chronic lymphocytic leukemia (B-CLL): results of training & test studies.
Cesano A, Perbellini O, Evensen E, Chioffi F, Ptacek J, Cignola R, Cordeiro J, Hawtin RE, Ware J, Cavallini C, Pizzolo G, and Scupoli MT.
Presented at: 2nd World Congress of Controversies in Hematology; September 6–8, 2012; Barcelona, SP.

Assessing signaling pathways associated with in vitro resistance to cytotoxic agents in AML.
Rosen DB, Cordeiro JA, Cohen A, Lacayo N, Hogge D, Hawtin RE, and Cesano A.
Leuk Res 2012;36:900-904.

Racial differences in B cell receptor signaling pathway activation.
Longo DM, Louie B, Mathi K, Pos Z, Wang E, Hawtin RE, Marincola FM, and Cesano A.
J Transl Med 2012;10:113.

Single Cell Network Profiling (SCNP) assay application to bladder cancer samples: identifying rare EGF responsive epithelial cancer cells sensitive to PI3K inhibition from bladder cancer washings.
Okhunov Z, Covey TM, Gulrajani M, Levey H, Cholankeril M, Marimpietri C, Fields S, Cesano A, and Vira M.
Presented at: Annual Meeting of the American Urological Association; May 19–23, 2012; Atlanta, GA.

Single cell network profiling (SCNP) assay allows for the identification of heterogeneity in the activation of multiple signaling pathways in distinct human B cell subsets.
Longo D, Conroy A, Louie B, Wang E, Pos Z, Marincola F, Hawtin R, and Cesano A.
Presented at: 99th Annual Meeting of the American Association of Immunology; May 4–8, 2012; Boston, MA

Leveling the playing field: bringing development of biomarkers and molecular diagnostics up to the standards for drug development.
Poste G, Carbone DP, Parkinson DR, Verweij J, Hewitt SM, and Jessup JM.
Clin Cancer Res 2012;18:1515-1523.

Single-cell network profiling of peripheral blood mononuclear cells from healthy donors reveals age- and race-associated differences in immune signaling pathway activation.
Longo DM, Louie B, Putta S, Evensen E, Ptacek J, Cordeiro J, Wang E, Pos Z, Hawtin RE, Marincola F, and Cesano A.
J Immunol; Prepublished online 13 January 2012; doi:10.4049/jimmunol.1102514.

Functional pathway analysis in acute myeloid leukemia using single cell network profiling assay: effect of specimen source (bone marrow or peripheral blood) on assay readouts.
Cesano A, Rosen DB, O'Meara P, Putta S, Gayko U, Spellmeyer DC, Cripe LD, Sun Z, Uno H, Litzow MR, Tallman MS, and Paietta E.
Cytometry Part B 2012;82B:158-172.

Single cell network profiling (SCNP)-based classifier to predict response to induction therapy in pediatric patients with de novo acute myeloid leukemia (AML): validation study results.
Lacayo NJ, Alonzo TA, Gayko U, Westfall M, Purvis N, Putta S, Hackett J, Cohen AC, Gerbing RB, Dahl GV, Bamis AS, and Meshinchi S.
Presented at: 53rd Annual Meeting of the American Society of Hematology; December 10–13, 2011; San Diego, CA.

BCR responsiveness is associated with time to first treatment (TTFT) in B-cell chronic lymphocytic leukemia (B-CLL): results from a single cell network profiling (SCNP) verification study.
Cesano A, Evensen E, Ptacek J, Cordeiro J, Hawtin RE, Ware JR, Nichele I, and Scupoli MT.
Presented at: 53rd Annual Meeting of the American Society of Hematology; December 10–13, 2011; San Diego, CA.

Single cell network profiling (SCNP) functionally characterizes FLT3 pathway deregulation in non-M3 acute myeloid leukemia (AML) and provides prognostic value independent from mutational status.
Cesano A, Putta S, Mathi K, Rosen DB, Gayko U, Hawtin RE, Cripe LD, Sun Z, Tallman MS, and Paietta E.
Presented at: 53rd Annual Meeting of the American Society of Hematology; December 10–13, 2011; San Diego, CA.

Single cell network profiling (SCNP) reveals age- and disease-based heterogeneity in healthy individuals and in patients with low risk (LR) myelodysplastic syndrome (MDS).
Cohen AC, Huang Y-W, Cesano A, Hawtin RE, Ware JR, Arulrajah B, and Kornblau SM.
Presented at: 53rd Annual Meeting of the American Society of Hematology; December 10–13, 2011; San Diego, CA.

Single cell network profiling (SCNP) reveals race-associated differences in B cell receptor signaling pathway activation.
Longo D, Louie B, Mathi K, Pos Z, Wang E, Hawtin RE, Marincola F, and Cesano A.
Presented at: 53rd Annual Meeting of the American Society of Hematology; December 10–13, 2011; San Diego, CA.

Impact of time from blood draw to peripheral blood mononuclear cell (PBMC) processing and cryopreservation as functional pathway activity measured by single cell network profiling (SCNP) assays.
Longo D, Louie B, Evensen E, Hawtin RE, and Cesano A.
Presented at: 53rd Annual Meeting of the American Society of Hematology; December 10–13, 2011; San Diego, CA.

Single cell network profiling (SCNP) assay application to bladder cancer (BC) samples: Identifying rare epidermal growth factor (EGF) responsive epithelial cancer cells sensitive to PI3K inhibition from bladder washings.
Covey T, Vira M, Gulrajani M, Cholankeril M, Levey HR, Marimpietri C, Cesano A, and Fields SZ.
Presented at: AACR-NCI-EORTC Molecular Targets and Cancer Therapeutics International Conference; November 12–16, 2011; San Francisco, CA.

Assessing specific biological mechanisms of drug resistance in adult and pediatric AML.
Rosen DB, Cordeiro J, Cohen AC, Lacayo N, Hogge D, Cesano A, and Hawtin R.
Presented at: AACR-NCI-EORTC Molecular Targets and Cancer Therapeutics International Conference; November 12–16, 2011; San Francisco, CA.

Integrating in vitro signaling and drug response data from single cell network profiling (SCNP) to inform on individual's AML biology.
Covey T, Gulrajani M, Hawtin R, Gayko U, Cumpston A, Craig M, Hamadani S, Rishel A, Park J, Abraham J, Remick S, and Tse W.
Presented at: AACR-NCI-EORTC Molecular Targets and Cancer Therapeutics International Conference; November 12–16, 2011; San Francisco, CA.

Association of reactive oxygen species-mediated signal transduction with in vitro apoptosis sensitivity in chronic lymphocytic leukemia B cells.
Palazzo AL, Evensen E, Huang Y-W, Cesano A, Nolan GP, and Fantl WJ.
PLoS One 2011;6(10):e24592. doi:10.1371/journal.pone.0024592.

Using Single-cell Network Profiling (SCNP) signatures to predict response to induction therapy and relapse risk in paediatric patients with acute myeloid leukemia (AML).
Cesano A, Westfall M, Rosen D, Louie B, Meshinchi S, Alonzo T, Arceci R, Ravindranath Y, Dahl G, and Lacayo N.
Presented at: The European Multidisciplinary Cancer Congress; September 23–7, 2011; Stockholm, Sweden.

Poly(ADP-ribose) polymerase-1 inhibition: preclinical and clinical development of synthetic lethality.
Leung M, Rosen D, Fields S, Cesano A, and Budman DR.
Mol Med 2011;17:854-862.

Mapping immune cell signaling network responses using single cell network profiling in peripheral blood mononuclear cells from healthy donors: defining “normal”.
Longo D, Louie B, Ptacek J, Cordeiro J, Pos Z, Wang E, Marincola F, and Cesano A.
Presented at: Annual meeting of the Clinical Immunology Society; May 19–22, 2011; Chicago, IL.

Single-cell network profiling (SCNP) to evaluate the proteomic profiles associated with ON 01910.Na treatment of MDS patients (pts).
Cesano A, Soper D, Banville S, Tran M, Seetharam M, Jumar R, Wilhelm F, and Greenberg P.
Presented at: 102nd Annual Meeting of the American Association for Cancer Research; April 2–6, 2011; Orlando, FL.

Signaling changes in the stem cell factor–AKT-S6 pathway in diagnostic AML samples.
Kornblau SM, Covey T, Putta S, Cohen A, Woronicz J, Fantl WJ, Gayko U, and Cesano A.
Blood Cancer Journal. (2011) 1, e3; doi:10.1038/bcj.2010.2.

Beyond sequencing: new diagnostic tests turn to pathways.
Garber, K.
JNCI J Natl Cancer Inst. 2011;103(4):290-2.

Single cell network profiling (SCNP) signatures predict response to induction therapy and relapse risk in pediatric patients with acute myeloid leukemia: Children's Oncology Group (COG) study POG-9421.
Lacayo NJ, Cohen A, Westfall M, Lackey A, Xin X, Gayko U, Putta S, Meshinchi S, Raimondi S, Alonzo TA, Arceci RJ, Ravindranath Y, Van Houten Dahl G, and Cesano A.
Presented at: 52nd Annual Meeting of the American Society of Hematology; December 4–7, 2010; Orlando, FL.

Single cell network profiling (SCNP) assay reveals B cell receptor signaling heterogeneity in the context of ZAP-70 expression in patients with chronic lymphocytic leukemia (CLL).
Shults K, Evensen E, Scupoli MT, Zanotti R, Xin X, and Cesano A.
Presented at: 52nd Annual Meeting of the American Society of Hematology; December 4–7, 2010; Orlando, FL.

Characterization of FLT3 pathway deregulation by single cell network profiling (SCNP) stratifies AML patients beyond molecular profiling.
Rosen DB, Putta S, Gayko U, Cesano A, Cripe L, Sun Z, Litzoe MR, Uno H, Tallman MS, and Paietta E.
Presented at: 52nd Annual Meeting of the American Society of Hematology; December 4–7, 2010; Orlando, FL.

Single cell network profiling as a platform to reveal leukemia-specific signaling signatures and sensitivity to kinase inhibitor therapies.
Covey TM, Gulrajani M, Becker H, Chandler JC, Schwind S, Marcucci G, and Cesano A.
Presented at: 52nd Annual Meeting of the American Society of Hematology; December 4–7, 2010; Orlando, FL.

Single-cell network profiling (SCNP) signatures independently predict response to induction therapy in older patients with acute myeloid leukemia (AML).
Cesano A, Putta S, Gayko U, Hackett J, Rosen DB, Cripe L, Sun Z, Uno H, Litzow MR, Tallman MS, and Paietta E.
Presented at: 52nd Annual Meeting of the American Society of Hematology; December 4–7, 2010; Orlando, FL.

Specimen source (BM or PB) does not affect proteomic signaling in patients with AML and circulating blasts.
Cesano A, Rosen DB, Putta S, Gayko U, Cripe L, Sun Z, Uno H, Litzow MR, Tallman MS, and Paietta E.
Presented at: 52nd Annual Meeting of the American Society of Hematology; December 4–7, 2010; Orlando, FL.

Cellular and intracellular network characterization of cytokine JAK/STAT signaling in whole blood across multiple healthy individuals: defining “normal”.
Covey TM, Gulrajani M, Marimpietri B, Chang AP, Panganiban-Lustan L, Cordeiro J, Longo D, Rosen D, and Cesano A.
Presented at: 52nd Annual Meeting of the American Society of Hematology; December 4–7, 2010; Orlando, FL.

Sample cryopreservation does not affect functional read outs in SCNP assays: implications for biomarker development.
Cesano A, Gotlib J, Lacayo NJ, Putta S, Lackey A, Gayko U, and Kornblau SK.
Presented at: 52nd Annual Meeting of the American Society of Hematology; December 4–7, 2010; Orlando, FL.

Single cell network profiling (SCNP) of IFN-α signaling pathways in peripheral blood mononuclear cells from healthy donors: implications for disease characterization, treatment selection, and drug discovery.
Longo DM, Evensen E, Fantl WJ, Pos Z, Marincola F, and Cesano A.
Presented at: 52nd Annual Meeting of the American Society of Hematology; December 4–7, 2010; Orlando, FL.

Single-Cell Network Profiling (SCNP) by flow cytometry in autoimmune disease.
Covey TM, Cesano A, and Parkinson DR.
Autoimmunity. 2010;43:550-9.

Functional characterization of FLT3 receptor signaling deregulation in acute myeloid leukemia by single cell network profiling (SCNP).
Rosen DB, Minden MD, Kornblau SM, Cohen A, Gayko U, Putta S, Woronicz J, Evensen E, Fantl WJ, and Cesano A.
Plos One. 2010;5(10):e13543.

Single cell network profiling by flow cytometry as a platform to reveal pathway signatures and drug response profiles for use in personalized medicine.
Covey TM, Gulrajani M, Soper D, and Cesano A.
Presented at: 4th Annual AACR International Conference on Molecular Diagnositcs in Cancer Therapeutic Development; September 27–30, 2010; Denver, CO.

Immune system networks and their reorganization in rheumatoid arthritis.
Ptacek J, Hale MB, Khalili H, Batliwalla F, Aranow C, Mackay M, Diamond B, Gregersen PK, and Nolan GP.
Presented at: 14th International Congress of Immunology; August 22–7, 2010; Kobe, JP.

Distinct patterns of DNA damage response and apoptosis correlate with JAK/STAT and PI3Kinase response profiles in human acute myelogenous leukemia.
Rosen DB, Putta S, Covey T, Huang Y-W, Nolan GP, Cesano A, Minden MD, and Fantl WJ.
Plos One. 2010;5(8):e12405.

Dynamic single cell network profiles in AML are associated with patient response to standard induction therapy.
Kornblau SM, Minden MD, Rosen DB, Putta S, Cohen A, Covey T, Spellmeyer DC, Fantl WJ, Gayko U, and Cesano A.
Clin Cancer Res. 2010;16:3721-33.

Single cell network profiling (SCNP) offers a novel approach to identify at diagnosis AML chemotherapy resistant cell phenotypes.
Cohen A, Covey T, Putta S, Gayko U, Xiaohua X, Fantl W, Kornblau S, and Cesano A.
Presented at: 15th Congress of the European Hematology Assocation; June 10–13, 2010; Barcelona, SP.

BCR signaling profiles associated with prognosis and progression in B-CLL.
Scupoli MT, Perbellini O, Cioffi F, Chignola R, Evensen E, Zanotti R, Nichele I, Lovato O, and Pizzolo G.
Presented at: 15th Congress of the European Hematology Assocation; June 10–13, 2010; Barcelona, SP.

Biological characterization of AML at the single cell level based on response to DNA damaging agents.
Rosen D, Minden M, Putta S, Covey T, Huang Y-W, Cesano A, Nolan G, and Fantl W.
Presented at: 15th Congress of the European Hematology Assocation; June 10–13, 2010; Barcelona, SP.

Dynamic single cell network profiles (SCNP) in AML are associated with patient response to standard induction therapy.
Cesano A, Minden M, Rosen D, Putta S, Cohen A, Fantl W, Gayko R, and Kornblau S.
Presented at: 15th Congress of the European Hematology Assocation; June 10–13, 2010; Barcelona, SP.

Single Cell Network Profiling (SCNP): mapping drug and target interactions.
Covey TM, Putta S, and Cesano A.
Assay Drug Dev Technol. 2010;8:321-43.

Informatics platform and workflows for robust high throughput single cell network profiling (SCNP).
Putta S, Spellmeyer D, Evensen E, Banville S, Friedland B, Rosen D, Soper D, Purvis N, Covey T, Francis-Lang H, and McCarthy J.
Presented at: 25th Congress of the International Society for the Advancement of Cytometry; May 8–12, 2010; Seattle, WA.

A comparative analysis of quantitative techniques using flow and mass cytometric platforms.
Purvis N, Ornatsky O, Shults K, Baranov V, Tanner, S, and Stelzer G.
Presented at: 25th Congress of the International Society for the Advancement of Cytometry; May 8–12, 2010; Seattle, WA.

Reproducibility of single cell network profiling using multiparameter flow cytometry.
Evensen E, Palazzo A, Gulrajani M, Purvis N, Banville S, Putta S, Gayko U, and Fantl W.
Presented at: 25th Congress of the International Society for the Advancement of Cytometry; May 8–12, 2010; Seattle, WA.

Towards robust evaluation of gating in flow cytometry.
Putta S.
Presented at: 25th Congress of the International Society for the Advancement of Cytometry; May 8–12, 2010; Seattle, WA.

The revolution is here - are you ready?
Parkinson DR.
EJHP Practice. 2010;16:12.

Identification of distinct apoptosis and myeloid signaling profiles within acute myeloid leukemia (AML) blast subpopulations.
Rosen DB, Minden MD, Putta S, Covey T, Huang Y-W, Cesano A, Nolan GP, and Fantl WJ.
Presented at: 101st Annual Meeting of the American Association for Cancer Research; April 17–21, 2010; Washington, DC.

Insights into acute myeloid leukemia via single cell network profiling.
Kornblau A, Minden SM, Hogge D, Cohen A, and Cesano A.
CLi. 2010;34:12-5.

Classification of acute myeloid leukemia (AML) based on apoptosis and myeloid signaling networks.
Rosen DB, Minden MD, Putta S, Covey T, Huang YW, Cesano A, and Fantl WJ.
Presented at: 51st Annual Meeting of the American Society of Hematology; December 5–8, 2009; New Orleans, LA.

Single cell network profiling (SCNP) to evaluate the mechanism of action of ON 01910.Na, a novel clinical trial stage compound.
Soper DM, Huang Y-W, Wilhelm F, Cosenza SC, Reddy EP, Cesano A, Greenberg PL, and Fantl WJ.
Presented at: 51st Annual Meeting of the American Society of Hematology; December 5–8, 2009; New Orleans, LA.

Single cell network profiling as a tool to identify AML chemotherapy resistant cell phenotypes under in vivo therapeutic pressure.
Cesano A, Woronicz J, Cohen AC, Covey T, Putta S, Gayko U, Fantl WJ, and Kornblau SM.
Presented at: 51st Annual Meeting of the American Society of Hematology; December 5–8, 2009; New Orleans, LA.

Distinct signaling profiles of gemtuzumab ozogamicin responsiveness and refractoriness in acute myeloid leukemia.
Rosen DB, Cordeiro JA, Soper DM, Huang Y-W, Hogge DE, Walter RB, Cesano A, and Fantl WJ.
Presented at: 51st Annual Meeting of the American Society of Hematology; December 5–8, 2009; New Orleans, LA.

Cost burden analysis of ineffective induction chemotherapy in elderly patients with AML.
Cohen AC, Davidson N, Scharf J, and Middlebrook D.
Abstract presented at: 51st Annual Meeting of the American Society of Hematology; December 5–8, 2009; New Orleans, LA.

FLT3-ITD signaling profiles in AML samples harboring mutations.
Minden MD, Kornblau SM, Rosen DB, Cohen AC, Gayko U, Putta S, Woronicz J, Fantl WJ, and Cesano A.
Blood (ASH Annual Meeting Abstracts). 2009;114:Abstract 1588.

Quantitative assessment of myeloid nuclear differentiation antigen distinguishes myelodysplastic syndrome from normal bone marrow.
Head HR, McClintock-Treep SA, Flye-Blakemore L, Jagasia M, Shinar A, Dupont WD, Stelzer G, Briggs R, and Shults KE.
Presented at: 51st Annual Meeting of the American Society of Hematology; December 5–8, 2009; New Orleans, LA.

Single cell network profiles in non-M3 AML associated with patient response to standard induction therapy.
Kornblau SM, Minden MD, Rosen DB, Putta S, Cohen AC, Covey T, Fantl WJ, Gayko U, and Cesano A.
Presented at: 51st Annual Meeting of the American Society of Hematology; December 5–8, 2009; New Orleans, LA.

Single cell profiling of B cell receptor signaling and apoptosis networks in chronic lymphocytic leukemia: association with in vitro sensitivity to fludarabine monophosphate (F-ara-A).
Evensen E, Palazzo A, Huang Y-W, Cesano A, Rassenti LZ, Kipps TJ, and Fantl WJ.
Presented at: 51st Annual Meeting of the American Society of Hematology; December 5–8, 2009; New Orleans, LA.

Single cell network profiling (SCNP) by flow cytometry as a tool to measure potency and selectivity of JAK/STAT inhibitors in PBMC and whole blood discrete cell subsets.
Covey TM, Putta S, Gulrajani M, Cohen AC, and Cesano A.
Presented at: AACR-NCI-EORTC Molecular Targets and Cancer Therapeutics International Conference; October 19–23, 2009; Boston, MA.

Educating for personalized medicine: a perspective from oncology.
Parkinson DR and Ziegler J.
Clinical Pharmacology And Therapeutics. July 2009;86(1):23-25.

Patient-specific classifications of human malignant disease.
Parkinson DR and Cesano A.
Curr Opin Mol Ther. 2009;11:252-9.

Distinct proteomic signaling networks stratify response to ara-C based induction therapy in patients with acute myeloid leukemia (AML).
Minden M, Rosen D, Putta S, Cohen A, Francis-Lang H, Covey T, Woronicz J, Soper D, Long B, Cordeiro J, Panganiban-Lustan L, Banville S, Gayko U, Cesano A, and Fantl W.
Presented at: 50th Annual Meeting of the American Society of Hematology; December 6–9, 2008; San Francisco, CA.

High level phosphatase activity revealed in chronic lymphocytic leukemia cells that use mutated immunoglobulin heavy chain variable region genes and lack high-level expression of the zeta-associated protein 70 (ZAP-70).
Fantl WJ, Yafawi RL, Putta SK, Huang Y-W, Francis-Lang HL, Laura RZ, Cohen AC, and Kipps TJ.
Presented at: 49th Annual Meeting of the American Society of Hematology; December 8–11, 2007; Atlanta, GA.

What's wrong with drug screening today.
Nolan GP.
Nat Chem Biol. 2007;3:187-191.

Mapping normal and cancer cell signalling networks: towards single-cell proteomics.
Irish JM, Kotech N, and Nolan GP.
Nature Rev Cancer. 2006;6:146-55.

Single cell profiling of potentiated phospho-protein networks in cancer cells.
Irish JM, Hovland R, Krutzik PO, Perez OD, Bruserud Ø, Gjertsen BT, and Nolan GP.
Cell. 2004;118:217-28.